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KMID : 0903519950380020168
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 2 p.168 ~ p.173
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Effects of Endosulfan on Cytochrome P - 450 Enzymes in Mouse(Balb/c .)
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Abstract
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To investigate the effects of endosulfan on cytochrome P-450 enzymes in mouse(Balb c.), endosulfan was given by an intraperitoneal dose of 7.5 ㎎/㎏. The treatment of endosulfan increased the cytochrome P-450 content by 3.3 to 42 fold, cytochrome b_5 content by 2.3 to 3.8 fold, NADPH cytochrome P-450 reductase activity by 5.3 to 6.4 fold and total haem content by 3.1 to 3.6 fold of mouse liver after 48 hrs of intraperitoneal injection. Endosulfancytochrome P-450 absorption spectrum exhibited miximum at 387㎚ and 389 ㎚ and broad near 407 ㎚ in the liver microsome. Reduced P-450-CO spectrum of the liver microsome exposed by the treatment of endosulfan showed maximum at 449 ㎚ and 450 ㎚ compared to that of the control having maximum at 451 ㎚, which indicated endosulfan induced cytochrome P-450 new isozymes. Aldrin epoxidase activities in the mouse liver and kidney were increased by 2.8 and 2.1 fold by the treatment of endosulfan. Also 7-ethoxyresorufin dealkylase activities in the mouse liver and kidney were elevated by 1.7 and 1.8 fold by treatment of endosulfan.
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KEYWORD
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